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| Fernandes,Annielle Regina Fonseca; Azevedo,Sérgio Santos de; Piatti,Rosa Maria; Pinheiro,Eliana Scarcelli; Genovez,Margareth Élide; Azevedo,Adílio Santos de; Batista,Carolina de Sousa Américo; Alves,Clebert José. |
| To determine the frequency of anti-Brucella canis antibodies in dogs attended in veterinary clinics from Patos, Paraíba State, Brazil, as well as to identify risk factors and to isolate and identify the agent, 193 dogs were used. Agar gel immunodiffusion test (AGID) was used to detect B. canis antibodies in sera. Isolation of B. canis was carried out in blood and bone marrow from seropositive animals. Six animals tested seropositive in AGID, resulting in a frequency of 3.11%. B. canis was isolated from bone marrow of one seropositive animal, with confirmation by PCR. Lack of cleaning of the dog's environment was identified as risk factor (odds ratio = 7.91). This is the first report of isolation of B. canis in dogs from the Northeast region of Brazil. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bacterial diseases; Canine brucellosis; Prevalence; Risk factors; Microbiological culture. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400023 |
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| Bunetel, L; Segui, A; Guerin, J; Lerman, I; Cormier, M. |
| The principle of this method is to evaluate, by counts exercised upon selective media, the relative importance of the different bacteria families in the flora of the coastal waters. It is important to separate the contaminant species and the commensal species in the marine environment. Ten culture media distributed into four categories (selective, non selective, saline and non saline) were examined by numeration. Counts were transferred on a diagram allowing to plot down a profile of bacterial flora of water sampled from a given geographical area. The profiles are arranged by a classification method. The sampling points with similar or adjoining profiles were assembled and marked on the chart. In this way, the authors obtained areas representing the impact... |
| Tipo: Text |
Palavras-chave: Bacteria; Coastal waters; Microbiological culture; Sewage disposal; Microbial contamination; Bacterial counters; Bacteria collecting devices. |
| Ano: 1984 |
URL: http://archimer.ifremer.fr/doc/1984/acte-1004.pdf |
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| Bruni, V; Crisafi, E; Maugeri, T; Zaccone, R. |
| 110 Vibrio strains were isolated, at various times of the year, from samples of sea-water supply, breeding-tank water and discarded fish used as food at the breeding stations. From the results obtained by the biochemical and cultural tests, it was possible to identify most of the isolated strains as Vibrio anguillarum . Strains with intermediate characteristics, above-all positive saccharose, proved to be numerous. The authors discuss the occurrence, in particular environments such as those being studied, of atypical or intermediate vibrio strains which are difficult to classify. |
| Tipo: Text |
Palavras-chave: Vibrio; Microbiological culture; Bacteria; Food; Minced products; Fish ponds; Sea water; Identification; Marine aquaculture; Bacteria collecting devices; Microbiological analysis. |
| Ano: 1984 |
URL: http://archimer.ifremer.fr/doc/1984/acte-997.pdf |
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| Kim, S; Hoppe, H. |
| A rapid and sensitive method to detect the extracellular enzymatic activity of bacteria colonies grown on agar plates is described. Selective agar media supplemented with protein, starch, chitin, Tween-80, etc. are conventionally used to detect biochemical properties of bacteria. It has been experimentally demonstrated with bacteria pure cultures that fluorogenic Methylumbelliferyl (MUF)-substrates are excellent substrate analogues for normally occurring polymers. Based on MUF-substrate hydrolysis the new method provides reliable qualitative estimates of extracellular enzymatic properties of bacteria within minutes using pure cultures as well as agar plates prepared for colony counts. |
| Tipo: Text |
Palavras-chave: Bacteria; Microbiological culture; Biochemical analysis; Enzymatic activity; Analytical techniques. |
| Ano: 1984 |
URL: http://archimer.ifremer.fr/doc/1984/acte-959.pdf |
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| Okpokwasili, G; Somerville, C; Grimes, D; Colwell, R. |
| A differential, phenanthrene-enrichment agar plating technique was used to isolate phenanthrene-degrading bacteria from phenanthrene-enriched Chesapeake Bay sediment. One of the isolates, a yellow pigmented, slime-producing, Gram-negative rod identified as Flavobacterium sp. has been studied in detail. It has been found to carry a single plasmid with a mass of about 34 megadaltons. Results of hydrocarbon adherence tests showed that the organism adhered only minimally to n-octane and n-hexadecane, but emulsified cyclohexylbenzene and 1,2,3,4,-tetrahydronaphthalene (tetralin). Curing of the plasmid with 3 mu g/ml novobiocin resulted in loss of phenanthrene clearing ability. Ability to degrade phenanthrene was confirmed using super(14)C-labeled phenanthrene. |
| Tipo: Text |
Palavras-chave: Flavobacterium; Microbiological culture; Culture media; Bacteria; Sediment pollution; Biodegradation; Bacteria collecting devices. |
| Ano: 1984 |
URL: http://archimer.ifremer.fr/doc/1984/acte-1014.pdf |
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